Cell culture - Serums - Media Biotium Cell viability and apoptosis markers Staining of living and dead cells

Staining of living and dead cells

Variants and accessories

Viability/cytotoxicity test kit

Nucleic acid markers - living cells

Stains live animal cells with green fluorescence and dead cells with red fluorescence for detection by fluorescence microscopy or flow cytometry
• Kit contenant 2 colorants : la calcéine AM et l'homodimère III d'éthidium
• Calcein-AM is a cell-permeable, non-fluorescent ester that can passively enter cells. In viable cells, it is hydrolysed by esterases in the cytoplasm to release the green fluorescent dye calcein. Calcein itself is impermeable to membranes and is only retained in viable cells with an intact plasma membrane
• Ethidium homodimer III is a red fluorescent nucleic acid dye that is impermeable to membranes. It selectively colours dead cells with damaged plasma membranes (necrotic or late apoptotic cells)

NucSpot® Live 488 and NucSpot® Live 650 nucleic acid stains specifically stain the nuclei of living or fixed cells
• Présentent une excellente spécificité pour l'ADN sans nécessiter d'étape de lavage, avec une faible toxicité pour l'imagerie des cellules vivantes
• NucSpot® Live 650 compatible with super-resolution SIM and STED imaging

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